DENSsolutions successfully installs another Climate system in Japan, at JFCC

DENSsolutions successfully installs another Climate system in Japan, at JFCC

Top row – from left to right: Mr. Suzuki (Nano Tech Solutions), Mr. Anada (JFCC), Dr. Lars van der Wal (DENSsolutions) and Mr. Hirai (JEOL). Bottom row – from left to right: Mr. Fukunaga (JEOL), Mr. Jinbo (JEOL) and Mr. Hisada (JEOL).

We are proud to announce that DENSsolutions has installed another Climate system in Japan, at the Japan Fine Ceramics Center, located in Nagoya, a highly populated Japanese port city. In this article, we interview Dr. Satoshi Anada, Senior Researcher at the Nanostructures Research Lab in JFCC, to learn more about their microscopy facility, its research direction, as well as how our Climate system is advancing their research.

Can you tell me about Japan Fine Ceramics Center and its research and development initiatives?

Japan Fine Ceramics Center (JFCC) was established back in 1985, with the goal of improving the quality of fine ceramics mainly through integrated testing and evaluation systems. JFCC has numerous business activities, one of which is the research and development (R&D) of materials, manufacturing technology and evaluation technology. Our R&D initiatives are focused on obtaining technological solutions to problems related to the environment, energy and safety. We have two main laboratories: 1) the Materials R&D Lab and 2) the Nanostructures Research Lab. The Materials R&D Lab focuses on the development of highly functional and novel materials (mainly ceramics) by improved process control, whereas the Nanostructures Research Lab focuses on the development and enhancement of state-of-the-art electron microscopy and related technologies. At the Nanostructures Research Lab, we have a high-end electron microscope – the JEOL JEM-ARM300F2 Grand ARM. This microscope enables us to observe samples at ultra-high spatial resolution with highly sensitive analysis over a wide range of accelerating voltages.”

What type of applications are the users at the Nanostructures Research Lab interested in using the Climate G+ system for?

“Users at the Nanostructures Research Lab are interested in applying the DENSsolutions Climate system to record operando TEM observations of battery and catalyst materials. We aim to understand where and how reactions take place, and which conditions enhance the performance of those materials. Moreover, we are interested in the electrochemical oxidation of materials in reaction with oxidants such as oxygen and hydrogen.”

What particular features of the DENSsolutions Climate G+ system attracted you to the system? 

“In order to understand factors and mechanisms related to the performance of battery and catalyst materials, it is important to observe their reactions in the actual environments in which they are used. The Climate system has the ability to flexibly and rapidly adjust gas composition, temperature, flow and pressure, which enables us to observe our battery and catalyst materials under various experimental conditions. This is capability is particularly what attracted us to the solution.”

In your experience so far, how have you found working with the Climate G+ system?

“The preliminary processes including the assembly of the Climate Nano-Reactor and leak testing are quite straightforward, assisted by the well-established Climate manual and software. With the Climate system, we have been able to perform numerous experiments without running into any leakage issues. Moreover, we are particularly impressed with the stability of the system even at extremely high temperatures.”

Dr. Satoshi Anada
Senior Researcher | Japan Fine Ceramics Center

Dr. Satoshi Anada received his Ph.D. degree in Engineering, Material Science, from Osaka University. Previously, he was working as a Specially Appointed Assistant Professor in the Research Center for Ultra-High Voltage Electron Microscopy at Osaka University. Currently, Dr. Anada is working as a Senior Researcher in the Japan Fine Ceramics Center (JFCC). His research was focused on the electromagnetic analysis of functional materials and devices using transmission electron microscopy, and now particularly on different microscopic measurement informatics.

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Meet Dr. Evgeniya Pechnikova, our new Senior Applications Development Scientist

Meet Dr. Evgeniya Pechnikova, our new Senior Applications Development Scientist

We are happy to announce that DENSsolutions has expanded the team with a wonderful new colleague – Dr. Evgeniya Pechnikova.

In the pursuit to grow our applications development for the DENSsolutions Stream system and other future product lines, we recently welcomed Dr. Evgeniya Pechnikova to the team – our new Senior Applications Development Scientist. Evgeniya has years of experience and high-level technical expertise in electron microscopy (EM), including cryogenic-EM, tomography and data processing techniques. Her role at DENSsolutions will be focused on developing novel in situ liquid EM applications that demonstrate the power of our solutions. She will also be working on creating new techniques and methodologies that will support the research of our customers, as well as perfecting our demonstration and installation protocols. We are thrilled to have Evgeniya join the DENSsolutions family and look forward to the valuable contributions she will make to the team on both a personal and professional level. In this article, we have asked Evgeniya to introduce herself so you can learn more about her extensive experience and her new role at DENSsolutions.

Meet Dr. Evgeniya Pechnikova

“My name is Evgeniya Pechnikova, and I was born and raised in Moscow, Russia. I have a lot of love for adventure and history, and enjoy travelling around the world to explore ruins of ancient civilizations. I’m also an avid fan of photography, Latin social dances like salsa and tango, as well as playing badminton and a game known as ‘werewolves’!

I have always been drawn to different spheres of science. That is why for my higher education, I decided to enrol in Lomonosov Moscow State University‘s biological faculty. As they had a very broad program there, I had the opportunity to learn about a wide range of subjects, starting with mathematics and physics and ending up with biochemistry, plant physiology and even drawing.

During my master’s degree, I was introduced to the world of electron microscopy and was completely fascinated by its combination of science and photography – two topics that have always been a passion of mine. To add another dimension to my work, I found a PhD position in the field of cryo-EM and 3D reconstruction, working on investigating the structure and function of capsids of a helical plant virus. My PhD project was a result of a collaboration between Lomonosov Moscow State University, Institute of Crystallography in Moscow and Birkbeck, University of London. During this PhD, I worked in close collaboration with structural biologists that were highly experienced in cryo-EM and image processing before the new detector technology popularised the technique to a broader base of scientists. This was an incredibly enriching experience and certainly shaped my career path moving forward.

After completing my PhD, I joined Thermo Fisher Scientific in the Netherlands where I worked as an Applications Scientist in the Eindhoven NanoPort facility – a state-of-the-art electron microscopy facility that provides users with hands-on experience with numerous analytical techniques. During this role, I was responsible for providing training and demonstrations for life science TEM equipment. I was also in frequent collaboration with the R&D department to translate customer needs into applications development, and to test new hardware and software tools. As a result of this work, I was able to play a role in accelerating the new-resolution revolution that has bloomed in cryo-EM over the past couple years. Importantly, this role made me realize that trying new tools to solve long-standing problems is what excites me most. 

Now, as a Senior Applications Development Scientist at DENSsolutions, I have the unique opportunity to combine and explore two cutting-edge technologies, MEMS and cryo-EM. My top priority is to boost applications development and create working protocols that showcase the advantages of DENSsolutions’ MEMS technology within in situ liquid EM. I look forward to being actively involved in hands-on support during on-site and remote product demonstrations, as well as working with other departments to translate customer needs into workable solutions. I am excited to take on this new challenge and am confident that I will be able to make a meaningful contribution to the company, our customers and science as a whole.”

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Meet our new Vice President of Sales and Business Development, Hans Radhoe

Meet our new Vice President of Sales and Business Development, Hans Radhoe

We are excited to announce the expansion of our team with our new colleague, Hans Radhoe.

Despite being a small company in the canal-ringed city of Delft, DENSsolutions has always been highly ambitious, with its sights set on disruptive growth and innovation. Keeping in line with this, we wanted to expand our team with someone who could lead our business development, with a focus on the discovery of new growth opportunities for the company and making our many visions a reality. We are happy to have found just the right person for this position – Hans Radhoe, our new Vice President of Sales and Business Development. With over 25 years of experience in business and development within fields such as life science and materials science, Hans has an extensive understanding of lead generation, advancing business strategy and the sales cycle process within diverse scientific markets. His role at DENSsolutions will focus on finding avenues of growth for the company and leading numerous aspects of the sales process, from prospecting and go-to-market strategies to customer relationship management. In this article, we have asked Hans to introduce himself so you can learn more about his education, experience and role at DENSsolutions. 

Meet Hans Radhoe

“My name is Hans Radhoe, and I was born in Suriname, a country in South America in the Amazon. At just 17 years old, I left Suriname with the bright intention to study medicine in the Netherlands. I had no knowledge at that time, however, that there was a lottery system in place in the Netherlands for being admitted into any of the medical schools in the country. As it turned out, I was not one of those selected to move forward in the admission process, which forced me to think of an alternative study to pursue.

This chain of events catapulted the start of my education in the field of biotechnology, which I studied at the Polytechnical Faculty in Delft. Studying biotechnology felt like an excellent choice for me since it not only piqued my interest, but was also a hot topic in the 80s and beyond. After finishing my Bachelor studies, my fascination with the environment led me to pursue a Master’s degree in Environmental Management at Open University in Heerlen, a small city in the southeast of the Netherlands bordering Germany.

After graduating, I took on a position for a couple years with the task of setting up a mycoplasma and virology lab in accordance with CGMP guidelines. In this role, I managed to upscale Mycoplasma testing services for the pharmaceutical industry. I then worked at Centocor in Leiden for a short while, which is a biotech subsidiary of Johnson & Johnson. During this role, I was responsible for the production of biologic drugs involving monoclonal antibody technology, such as Remicade – a drug used to treat a number of autoimmune diseases like rheumatoid arthritis. It was particularly during this role that I gained hands-on experience in cell and gene therapy. 

Shortly after, I made the switch from lab to business because I became more and more passionate about perceiving and fulfilling the commercial needs of others. During my business jobs, I realized how imperative it is to be able to listen intently and actively when in communication with customers. This encouraged me to take a Master’s in Counselling and Coaching, which is part of the Psychology study at the Radboud University Nijmegen. Although the intention of pursuing this Master’s was to use the knowledge learnt to enhance my communication with clients, I found myself applying it in the volunteer work that I did next to my job at the time. This volunteer work involved coaching cancer patients at the Nederlandse Federatie van Kankerpatiëntenorganisaties, which I happened to stumble upon when seeing an advertisement from the organisation on TV. 

Further down the line in my career, after many enriching vocational experiences, I started working as the Sales Director at Amsterdam Scientific Instruments (ASI) – a spin-off company from Nikhef, which is a Dutch research institute for particle physics. ASI specializes in the design and manufacturing of advanced detector solutions for all kinds of particles, such as electrons. Indeed, it was particularly during this position that I stepped into the world of electron microscopy, which I found to be rather fascinating.

This then brings me to today, where I now have the role of Vice President of Sales and Business Development at DENSsolutions, a company that develops advanced electron microscopy solutions. I have actually been following DENSsolutions for a long time since a friend of mine worked here, and I have witnessed for myself the rapid innovation of the company in the past few years. Generally, in the span of my career, I have been active in numerous scientific markets all over the world and in many different positions, including account management, sales and business development. In my new role, I will be in charge of increasing the company’s presence in existing markets, as well as forming strategic partnerships that open up entirely new ones. I look forward to applying my extensive knowledge gained over the past two decades into this new role, and being part of a fantastic team so focused on continuous cooperation and innovation.”

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DENSsolutions has installed yet another Climate system in the U.S. at Alfred University

DENSsolutions has installed yet another Climate system in the U.S. at Alfred University

We are proud to announce that DENSsolutions has installed another Climate system in the United States, at Alfred University, which is located in the west of New York State. In this article, we interview Dr. Kun Wang, Assistant Professor at the Inamori School of Engineering in Alfred University, to learn more about their microscopy facility, its research direction, as well as how our Climate system is advancing their research.

Can you tell me more about the microscopy facility at Alfred University?

Alfred University has numerous research facilities that boast a wide range of high-tech equipment. There are dedicated facilities for materials characterization, mechanical and physical testing, biological evaluation of materials, spectroscopy, materials synthesis and processing as well as imaging and microscopy. The Imaging and Microscopy facility is equipped with a scanning electron microscope, an atomic force microscope and a fluorescent optical microscope, among many other tools. Just last summer, we had our new transmission electron microscope installed, the TFS Talos F200X. This microscope is equipped with a super X-ray detector which enables us to perform high resolution chemical analyses in a highly efficient manner.”

What type of applications are the users at Alfred using the Climate system for?

“Users of the facility are interested in a couple of applications, now enabled via the use of our newly acquired DENSsolutions Climate system. Via Climate, we would like to perform in situ oxidation and reduction experiments on batteries and catalyst materials. Moreover, we are interested in performing in situ high-temperature oxidation experiments for aerospace materials and nuclear matter in order to better understand these materials and their behavior under varying temperature conditions. We are also interested in performing energy-dispersive X-ray spectroscopy (EDX) in those experiments to get a better idea of the elemental composition of a given sample.”

What particular features of the DENSsolutions Climate solution attracted you to the system?

“Aside from the ability of the system to combine gas and heating functions, it was particularly important for me to use an in situ system that could handle high temperatures. Specifically, I was looking for a system that could handle high temperatures while still maintaining the stability of the holder. This is particularly what attracted me most to the Climate system.”

Can you tell me about the grant that was won to acquire the system?

“The grant was actually awarded several years ago, from an institute called the New York State’s Empire State Development, which provides numerous services and resources for education, healthcare, military and other fields.”

DENSsolutions Prof. Jungwon Park

Dr. Kun Wang
Assistant Proffessor | Inamori School of Engineering, Alfred University

Dr. Kun Wang received his Ph.D. degree in Materials Science and Engineering from the Swiss Federal Institute of Technology Lausanne (EPFL). He used to work as a Postdoctoral Research Associate at the Nuclear Materials Science and Technology group of the Oak Ridge National Laboratory (ORNL). Currently, he is working as an Assistant Professor at the Inamori School of Engineering, Alfred University. His research focuses on study of structural materials under extreme environmental conditions.

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Introducing the Stream Liquid Supply System: An integrated solution offering unmatched flexibility

Introducing the Stream Liquid Supply System: An integrated solution offering unmatched flexibility

An interview with DENSsolutions Mechanical Engineer Alejandro Rozene about our latest addition to the Stream product line: the Liquid Supply System (LSS).

DENSsolutions introduces its latest solution: the Stream Liquid Supply System (LSS): an integrated solution designed to offer you ease-of-use, flexibility and reproducibility in your in situ liquid experiments. In this article, we interview our Mechanical Engineer Alejandro Rozene to learn all about the LSS, from what inspired its development, its unique capabilities and the many applications that will benefit from its creation.

What led to the development of the Stream Liquid Supply System?

“Our Liquid Phase Electron Microscopy (LPEM) solutions have been used by researchers around the world in a plethora of research fields, such as protein studies, battery research and molecular self-assembly. With our Stream system, users can fully control the microfluidic environment inside the Nano-Cell, our environmental MEMS sample carrier, while biasing or heating their sample. Considering the wide range of capabilities the system offers and the complexity of LPEM, Stream is quite the advanced system. This is precisely what inspired the next step in the innovation process: the development of an integrated solution designed to offer greater flexibility and ease-of-use. The Liquid Supply System (LSS) is a single scientific instrument that allows microscopists to carry out imaging experiments in fewer steps while introducing new features. The LSS is also designed to be the basic unit of a modular system that can be configured for different research applications.” 

What are the main benefits of the LSS for users?

“The introduction of the LSS to the Stream product line brings forth numerous advantages for your in situ liquid experiments, including greater flexibility, reliability and reproducibility. These benefits are detailed below:

1) Ease of use: Thanks to the clever architecture of the LSS, particularly the moveable base, you can easily relocate, store and set it up in various locations. It is also possible to control the microfluidic environment of the Nano-Cell and to apply different stimuli using a single interface in Impulse, our in situ experiment control and automation software. All of the sensor data is collected centrally. The LSS therefore simplifies the LPEM workflow and allows you to shift the focus from the hardware to the imaging experiment. This will potentially open the door to more elaborate experimental workflows.

2) High resolution imaging and meaningful analytical analysis: It is widely known that LPEM suffers from limited resolution caused by the thickness of the liquid layer inside the sample chamber. Even a 500 nm liquid layer can limit imaging resolution. With the LSS, the capability of controllable and inert gas purging is introduced. This means that you can easily displace the liquid in the Nano-Cell and effortlessly cycle between dry and liquid environments. This can be done with air or with an inert gas for air-sensitive samples. Via purging, you can easily get rid of excess liquid in the sample, thereby allowing you to achieve high resolution imaging, image in electron diffraction mode and perform spectroscopy techniques: EDS and EELS.

3) Reliable and reproducible results: The components of the LSS, such as the inlet and outlet pressure-based pumps as well as the liquid flow meter, introduce an unprecedented level of control during your liquid phase microscopy experiments. The LSS offers the unique ability to actively measure the liquid flow, making it possible for you to compare results from different experiments. Moreover, this means that you can easily detect potential clogs in the system and act fast, allowing you to spend your TEM time efficiently and effectively. Ultimately, the combination of our LSS and unique Nano-Cell design, having an on-chip inlet and outlet, enables the liquid delivery to be both reliable and reproducible with a success rate of more than 95%.”

Which applications will benefit most from the LSS?

“The LSS is beneficial for any researcher who wants to use LPEM to observe hydrated samples, matter suspended in liquid or liquid itself in a dynamic environment. Some of the many fields that will benefit from the system include:

  1. Life Science: DNA imaging, biomineralization, cell imaging and protein studies
  2. Electrochemistry: studies of aqueous electrolytes and electrocatalysis
  3. Material Science: studies of nanoparticle formation, self-assembly and growth

These are just a few examples of the several fields that can benefit from the Stream system. Furthermore, the door is open to any researcher who may want to exploit the unique capabilities of the LSS for any liquid-based study outside of the TEM.”

What kind of challenges were tackled during development?

“The LSS is a state-of-the-art scientific instrument. As with any other mechatronic development, integrating the sensing, actuating and microfluidic components into a single, robust machine was in itself a challenge. Also, our market is especially demanding since the users of this instrument already work with some of the most advanced equipment out there, which undoubtedly sets the bar very high. However, it was a very enjoyable experience thanks to the incredible teamwork and collaboration of our MEMS Project Manager, Tijn van Omme, and our Software Developer, Emil Svensson, as well as our manufacturing partners.”

What is the compatibility of the LSS?

“The LSS is an integral part of our Stream product line. It is therefore fully compatible with the modular Stream sample holder, the Stream liquid biasing and liquid heating Nano-Cells, as well as with Impulse. With regards to the microscopes, compatibility is given by the holder type. We currently offer compatibility with JEOL and Thermo Fisher Scientific microscopes.”

Has the LSS already been installed?

“Yes, the Liquid Supply System has been installed at the University of Alberta Nanofabrication and Characterization Facility (nanoFAB) in Canada. The nanoFAB is a national, open-access training, service, and collaboration centre, focused on academic and industrial applications in micro- and nanoscale fabrication and characterization. The installation was carried out by our MEMS Project Manager, Tijn van Omme, with the support of our close distributor Colt Murray from Nanoscience Instruments. It is the first of several installations planned in the upcoming months.”

From left to right: XueHai Tan, Colt Murray, Tijn van Omme and Haoyang Yu

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Meet our new colleagues Dr. Vasilis Papadimitriou and Erkin Demir

Meet our new colleagues Dr. Vasilis Papadimitriou and Erkin Demir

We are excited to announce the expansion of our team with two new wonderful colleagues: our MEMS Development Manager, Vasilis, as well as our Lab Manager and Quality Control Engineer, Erkin.

DENSsolutions Eva Bladt

A prime focus of DENSsolutions is the innovation and further development of our in situ microscopy solutions, including those catered to the life science community. With this goal in mind, we wanted to expand our team with someone who could lead the development of our MEMS technology for liquid phase transmission electron microscopy. Over the past 15 years, Vasilis has acquired a unique and diverse pool of knowledge within numerous fields, including micro-/nanotechnology, Lab-on-a-Chip devices and life science applications such as point-of-care diagnostics and wearable optical biosensors. His role at DENSsolutions is focused on the development of new MEMS devices which will enable advanced in situ microscopy solutions for the scientific community.

Aside from innovation, DENSsolutions recognizes the need to deliver top-quality products that not only meet but exceed customer expectations. This is precisely the value that Erkin will bring forth in light of his five-year experience in quality control at the leading automotive and aerospace companies in Turkey. Erkin will be responsible for the internal and supplier-side quality control activities at DENSsolutions, as well as the implementation of valuable quality control tools to DENSsolutions processes. We asked Vasilis and Erkin to introduce themselves so you can learn more about their education, experience and role at DENSsolutions. 

Meet Dr. Vasilis Papadimitriou

“My name is Vasilis Papadimitriou and I am 33 years old. I was born and raised in a small town called Levadia in Greece, and in 2013 I moved to the Netherlands.

In 2006, I started my Bachelor in Electronics and Computer Engineering at the Technical University of Crete in Greece. After that, I pursued a Master’s degree in the same field where I focused on programming, electronics and telecommunications. For my MSc thesis, I investigated the use of DNA for complex mathematical algorithms (DNA computing). After my military service, I moved to the Netherlands in 2013 and dived into the world of micro-/nanotechnology as part of my MSc in Electrical Engineering at the University of Twente. During this MSc thesis, I created a novel carbon electrode for use in supercapacitors, which granted me deep insights into electrochemistry and fluid mechanics. 

After that, I decided to continue my studies with a PhD in University of Twente. The focus of my doctorate was on Lab-on-a-Chip devices. I’ve always enjoyed multidisciplinary science, and Lab-on-a-Chip devices integrate numerous fields such as microtechnology, physics, chemistry, biology and electrical engineering. The European project that my PhD was part of focused on the development of a point-of-care device for early diagnosis of cardiovascular diseases. My role was to separate and concentrate specific proteins from a droplet of blood. In 2019, I received my doctorate degree and since then, I have had two postdoctoral positions: one on artificial intelligence for Lab-on-a-Chip applications at the University of Twente, and another on wearable optical biosensors at the Technical University of Delft.

That brings us to today, where I am really happy to be part of the DENSsolutions team. Actually, I wasn’t previously familiar with in situ microscopy nor the company, which is really unfortunate since their (and now our!) products could have solved many of the challenges I faced during my PhD and postdoctoral work. My main role at DENSsolutions is to investigate new technologies and develop intelligent MEMS devices that will make electron microscopy easier, faster and reproducible. With more than 15 years of experience within academia, I am very much well-acquainted with the struggles of research. I hope through DENSsolutions I will make the lives of scientists easier, while at the same time expanding my engineering and scientific horizons.”

Meet Erkin Demir

“My name is Erkin Demir. I am a 28-year-old individual of Circassian origin who was born and raised in Turkey. As of February 2022, I moved to the Netherlands for my exciting new role at DENSsolutions.

I started my Bachelor in Gazi University in the field of metallurgical and materials engineering. For my Bachelor’s thesis, I did research on the current and potential uses of graphene. After my undergraduate degree, I started working as a Quality Engineer in Bozankaya, a top manufacturer for public transportation vehicles in Turkey. There, I had the opportunity of managing the supplier deployment and incoming quality control processes of the first domestic 100% electric buses and trams. This job granted me my first exposure into the intricacies and importance of quality control for the delivery of top-quality products. As a next step in my career, I became a Quality Engineer at Erkunt Tractor Ind. Inc., an renowned tractor manufacturer in Turkey. This position helped me obtain deeper insights into field of quality control as a whole, as I was involved in areas such as mass production, lean manufacturing, supplier development, 5S and similar processes.

Afterwards, I landed a job as a Lead Quality Engineer at the Tusaş Engine Industry Inc., the leading aviation engine manufacturer in Turkey. During my work there, I was fully responsible for the quality side of the NPI processes of some parts in aircraft engine programs. I worked on the implementation of advanced measurement methods to ensure the high precision and safety conditions associated with the aviation industry. I obtained a lean 6 Sigma yellow belt and AS 13001 DPRV certification after completing related courses and exams. Thanks to this SAE authorization, I became eligible to sell aero engine parts on an international level. In parallel with this position, I completed my Master’s degree in Metallurgical and Materials Engineering at Gazi University in 2020. For my Master’s thesis, I examined the performance of high temperature diffusion coatings on the corrosion resistance of nickel-based superalloys used in high pressure turbine blades.

Here I am now, a different country, a different industry, and some awesome teammates. I couldn’t be more excited and curious to embark on this new journey. My main role at DENSsolutions is to ensure the proper functioning of all our products so that our customers can conduct their research without disturbance. This includes performing meticulous inspections and tests at our lab on our world-class MEMS devices, TEM holders and fluid handling systems. Moreover, I will be working very closely with suppliers to ensure the highest possible quality level for our products. I am excited to be deploying all the best practices I have learned over the years into DENSsolutions, adding value step by step.”

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