In situ dynamics in the liquid state
Ocean Application Fields
Cell & Cancer Biology
Biochemistry & Nanobiotechnology
Aggregation mechanisms for chemical and biomolecular sensing
20 nm Au nanoparticles were preloaded and real-time aggregation dynamics was observed in STEM mode as a function of pH. H. Su MSc & J. Xu TU Delft, The Netherlands Youku video
Aggregation & fusion mechanisms of polystyrene nanoparticles
Using unstained polystyrene particles suspended in water, imaging of the core-shell structure was recorded showing fusing of the particles from the ebeam interaction. Dr. N. Jose & Dr. C. Ducati Cambridge University, United Kingdom
Easy Sample Preparation
Directly deposit your sample onto the Nano-Cell
A pipette can be used for easy and fast loading of the sample onto the bottom chip of the Nano-Cell. For biological sample preparation, the bottom chip can be placed into a multi-well for cell culturing directly onto the chip.
Creating the liquid environment
within the microscope
The Ocean Nano-Cells are ultra-clean consumable sample carriers, which have electron transparent windows in the centre of the top and bottom chip (forming the Nano-Cell). A range of spacer sizes is available for any experiment and the low mechanical stress of high-quality SiNx makes each Nano-Cell robust.
Modular Sample Holder
Ensure a clean experiment
with a removable tip & tubing
Once the Nano-Cell is placed into self-aligning precision slot within the Sample Holder tip, the O-rings and lid are sealed and the system is ready for vacuum testing. As the tip is removable, the internal liquid tubing can be replaced at any time to avoid clogging / cross-contamination and can be cleaned. For STEM, the tip can be turned 180° to ensure that the sample is on the electron transparent window of the top chip.
The Complete Solution
Including Liquid syringe pump & Vacuum test station
Included in the Ocean system is the syringe pump which allows for a maximum flow rate of 10 ul/min and minimum flow rate of 25 nl/min through the Nano-Cell. Additionally, the vacuum test station ensures that, once the Sample Holder is leak tight, it is ready for inserting into the microscope.
Frequently Asked Questions
How much time does the preparation for an experiment take?
Preparing the system to run an experiment would typically take around 20 minutes:
- 5 minutes to load the sample on the Nano-Cell
- 5 minutes to assemble the tip: placing the Nano-Cell and closing off the lid
- 10 minutes to do the leak test
NOTE: This time does not include the sample preparation, which varies from sample to sample. Replacing the inner tubing would normally take around 15 minutes
Which liquids can be used?
Is it necessary to use the syringe pump?
Is it possible to record images of live eukaryotic cells with liquid STEM?
How can you maintain the cell viable in the electron beam and avoid considerable radiation damage?
Can I improve the resolution if I reduce the liquid layer?
What are the advantages of liquid (S)TEM over current ultrahigh-resolution optical methods (i.e. STED, PALM, STORM)?
Is it possible to do correlative microscopy between liquid STEM and other techniques such as fluorescent microscopy?
How to reduce the charging effects caused by the secondary electrons during STEM imaging of biosamples?
How can one mitigate the effect of thermal gradients and charging on non-conductive liquids?
How to prevent the sample from flowing away from the field of view?
Is it possible to tilt the holder?
Is it possible to heat or bias the liquid?
Download the Ocean brochure
For more information on workflow, applications and specifications.
Feel free to contact us with any further questions.
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